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--> Juntendo University, Tokyo, established in 1838. English Chinese Indonesian Korean Japanese About Juntendo Study Research Hospital information International Center Home Research Research Support Center Laboratory of Molecular and Biochemical Research Laboratory of Molecular and Biochemical Research  Introduction   Research   Publications   Laboratory  Japanese      Index Laboratory For details, please go to the "Laboratory" of the Juntendo portal site. Laboratory Map Location:Building 7, 5th Floor Training Area Here, you can learn molecular biological techniques from our staff. RNA preparation Reverse transcription reaction Conventional PCR Real-time PCR Microarray DNA sequencing Cell culture ELISA Cytotoxicity measurement Microelectrode array (MEA) Ca2+ imaging Other basic techniques for gene manipulation P2 level cell culture room(#5S05) Here, you can perform experiments using viruses, including adenovirus and retrovirus. Instruments available Safety Cabinet(2) (MHE-130AJ, Panasonic/NSE-1200ⅡB2, Dalton) CO2 Incubator (Model F370, Thermo / 9200E, 9300E, Waken) Low Speed Refrigerated Centrifuge (Himac CF7D2, Hitachi) High Speed Refrigerated Microcentrifuge (Himac CT13R, Hitachi) Fluorescent microscope (BZ-9000, Keyence) Microscope (TS-100, Nikon) Autoclave (SX-500, Tomy) Deep Freezer (-80℃) (MDE-U32V, Panasonic) Cell culture room (#5S11) Here, you can culture primary cells and established cell lines. Instruments available Clean benches (3) (Panasonic, JUJIFIELD, Showa kagaku) Safety Cabinet(2) (1345, Thermo /MHE-S901A2-PJ, Panasonic) CO2 Incubators (12) ( Model F370, Thermo / 9100Ex,9200Ex,Waken) Low Speed Refrigerated Centrifuge (Himac CF7D2, Hitachi) Fluorescent microscope (BZ-X800, Keyence) Microscopes (2) (Axiovert40, Primovert, Zeiss) Electroporator (3) (Nucleofactor II, Lonza / Neon, Thermo / NEPA Porator System 2, NEPAGENE) Microelectrode array system (MEA 2100-Systems, Bio Research Center) General BSL2 level experiment room(#5S) Here, you can perform biochemical and molecular biological experiments. Instruments available Safety Cabinet (AC2-3N7,ESCO) Microplate reader (SpectraMax 340PC384, Molecular Devices) Luminometer (2) (Lumat LB9507, Berthold for single test tube / SpectraMaxL, Molecular Device for 96-well plate) Thermal cycler (PCR apparatus) (2) (T-100, Bio Rad) Fluorescent microscope (2) (BZ-X700, Keyence) Trans Illuminator (Printgraph 2M, ATTO) UV-Vis Spectrophotometer (NanoDrop, Thermo) Automated electrophoresis system (TapeStation4150 , Agilent Technologies) P1A level room(#5S39) Here, you can sacrifice animals, such as mice and rats. Equipment room (#5S33) Here, you can use analysis instruments for molecular biology. Real-time PCR system (4) (7500 Fast Real time PCR systems, QuantStudio 3, Thermo) DNA sequencer(2) (3500/3730 Genetic Analyzer, Thermo) Next generation sequencer (MiniSeq system, Illumina) MicroArray system (GeneChip system, Thermo) Main Instruments Click here to view all instruments Real-time PCR system(Thermo Fisher Scientific)QuantStudio 3 Real-time PCR System,7500 Fast Real-Time PCR System Next generation sequencer (Illumina)MiniSeq system DNA sequencer (Thermo Fisher Scientific)3500 Genetic Analyzer,3730 Genetic Analyzer Microarray(Thermo Fisher Scientific)GeneChip system Automated electrophoresis system(Agilent Technologies)TapeStation4150 Microplate Reader(Molecular Devices)SpectraMax 340 PC384 Luminometer(Molecular Devices)SpectraMax L 2+ imaging system" width="220px"> Microelectrode array system(Bio Research Center)MEA 2100-Systems Ca2+ imaging system(Hamamatsu Photonics)AquaCosmos imaging system Fluorescent microscope(Keyence)BZ-X800,BZ-X700 Electroporator (NEPAGENE)NEPA Porator System2 (Thermo Fisher Scientific) Neon Transfection System (Lonza)Nucleofector Ⅱ Device Real-time PCR system(Thermo Fisher Scientific)QuantStudio 3 Real-time PCR System,7500 Fast Real-Time PCR System DNA sequencer (Thermo Fisher Scientific)3500 Genetic Analyzer,3730 Genetic Analyzer Microarray (Thermo Fisher Scientific)GeneChip system Automated electrophoresis system(Agilent Technologies)TapeStation4150 Microplate Reader(Molecular Devices)SpectraMax 340 PC384 Luminometer(Molecular Devices)SpectraMax L Microelectrode array system (Bio Research Center)MEA 2100-Systems Ca2+ imaging system (Hamamatsu Photonics) AquaCosmos imaging system Fluorescent microscope(Keyence) BZ-X800, BZ-X700 Electroporator (NEPAGENE) NEPA Porator System2 (Thermo Fisher Scientific) Neon Transfection System (Lonza) Nucleofector Ⅱ Device  Introduction   Research   Publications   Laboratory  Japanese     Index To Page Top Juntendo University Copyright &#64; 2011 JUNTENDO All Rights Reserved.

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